diff --git a/tests/extmod/uctypes_sizeof_layout.py b/tests/extmod/uctypes_sizeof_layout.py
new file mode 100644
index 0000000000..2108e81502
--- /dev/null
+++ b/tests/extmod/uctypes_sizeof_layout.py
@@ -0,0 +1,27 @@
+try:
+    import uctypes
+except ImportError:
+    print("SKIP")
+    raise SystemExit
+
+desc = {
+    "f1": 0 | uctypes.UINT32,
+    "f2": 4 | uctypes.UINT8,
+}
+
+
+# uctypes.NATIVE is default
+print(uctypes.sizeof(desc) == uctypes.sizeof(desc, uctypes.NATIVE))
+
+# Here we assume that that we run on a platform with convential ABI
+# (which rounds up structure size based on max alignment). For platforms
+# where that doesn't hold, this tests should be just disabled in the runner.
+print(uctypes.sizeof(desc, uctypes.NATIVE) > uctypes.sizeof(desc, uctypes.LITTLE_ENDIAN))
+
+# When taking sizeof of instantiated structure, layout type param
+# is prohibited (because structure already has its layout type).
+s = uctypes.struct(0, desc, uctypes.LITTLE_ENDIAN)
+try:
+    uctypes.sizeof(s, uctypes.LITTLE_ENDIAN)
+except TypeError:
+    print("TypeError")
diff --git a/tests/extmod/uctypes_sizeof_layout.py.exp b/tests/extmod/uctypes_sizeof_layout.py.exp
new file mode 100644
index 0000000000..281f20856e
--- /dev/null
+++ b/tests/extmod/uctypes_sizeof_layout.py.exp
@@ -0,0 +1,3 @@
+True
+True
+TypeError