diff --git a/ports/raspberrypi/boards/challenger_nb_rp2040_wifi/mpconfigboard.h b/ports/raspberrypi/boards/challenger_nb_rp2040_wifi/mpconfigboard.h
index 013b8e9276..a3f79af481 100644
--- a/ports/raspberrypi/boards/challenger_nb_rp2040_wifi/mpconfigboard.h
+++ b/ports/raspberrypi/boards/challenger_nb_rp2040_wifi/mpconfigboard.h
@@ -5,6 +5,3 @@
 #define DEFAULT_UART_BUS_RX   (&pin_GPIO17)
 #define DEFAULT_I2C_BUS_SDA   (&pin_GPIO0)
 #define DEFAULT_I2C_BUS_SCL   (&pin_GPIO1)
-#define DEFAULT_SPI_BUS_SCK   (&pin_GPIO22)
-#define DEFAULT_SPI_BUS_MOSI  (&pin_GPIO23)
-#define DEFAULT_SPI_BUS_MISO  (&pin_GPIO24)